Experimental evaluation of flexible duplexing in multi-tier MIMO networks

In this paper, we present an experimental evaluation of the performance benefits provided by flexible duplexing, an access technique that allows uplink and downlink cells to coexist within the same time-frequency resource blocks. In order to replicate a wireless multi-tier network composed of 1 macro-cell and 2 small cells, a measurement campaign has been conducted using an indoor wireless testbed comprised of a total of 6 multiple-input multiple-output (MIMO) software-defined radio (SDR) devices. Since each cell has a single active user, each uplink/downlink configuration can be identified with a different interference channel, over which interference alignment (IA) is used as an inter-cell interference management technique and compared to other existing methods. The obtained results show that flexible duplexing clearly outperforms the conventional time-division duplex (TDD) access approach, where all cells operate synchronized either in uplink or dowlink mode. Additionally, interference alignment consistently provides better results in most of the interference regimes when compared to minimum means quare error (MMSE)-based schemes. The impact of channel estimate quality on the different communication strategies is also studied. It is worth highlighting that the presented over-the-air (OTA) experiments represent the first implementation of IA with real-time precoding and decoding.The work of Jacobo Fanjul, Jesús Ibáñez and Ignacio Santamaria has been supported by the Ministerio de Economía, Industria y Competitividad (MINECO) of Spain, and AEI/FEDER funds of the E.U., under grant TEC2016-75067-C4-4-R (CARMEN), grant PID2019-104958RB-C43 (ADELE), and FPI grant BES-2014-069786. The work of José A. García-Naya has been funded by the Xunta de Galicia (ED431G2019/01), the Agencia Estatal de Investigación of Spain (TEC2016-75067-C4-1-R, RED2018-102668-T), and ERDF funds of the E.U. (AEI/FEDER, UE)

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

Experimental analysis of downlink rate performance in flexible duplexing wireless heterogeneous networks

RESUMEN: En este trabajo se presenta una serie de medidas realizadas en un entorno experimental, en el cual, se compara el rendimiento que otorga el uso de flexible duplexing en relación con el que presentan técnicas convencionales. Se ha hecho una revisión sobre el state of the art de las comunicaciones inalámbricas, haciendo énfasis en los sistemas MIMO, que están, hoy en día, entre las tecnologías que presentan mejores prestaciones en este tipo de comunicaciones. Además, se describen diferentes técnicas de comunicaciones que serán empleadas en este experimento, prestando principal atención a interference alignment. Dicha técnica, se centra exclusivamente en cancelación de interferencias, siendo una de las que mejor rendimiento presentan. A continuación, se describen los aspectos relacionados con el contexto en el que se desarrollará este experimento. Dicha descripción abarca las principales prestaciones de los dispositivos que serán empleados, distribución de las diferentes celdas que serán objeto de estudio y formato de la trama que será transmitida por los diferentes nodos. Se presentan resultados gráficos sobre las prestaciones, en términos de precisión a la hora de decodificar los símbolos recibidos, que presentan las diferentes técnicas de comunicaciones empleadas en las diferentes celdas que componen dicho experimento. Por último, se extrae una serie de conclusiones sobre este experimento y además, se establecen unas líneas futuras por las que continuar el trabajo de investigación.ABSTRACT: In this work, we present an experimental evaluation of flexible duplexing, a promising communication strategy in the context of heterogeneous networks (HetNets), and we compare its performance to conventional time-division schemes. The state of the art of wireless communications is studied, focusing on MIMO systems, which are considered to provide excellent performance in the context of 4G and 5G. Additionally, we introduce the transmission methods under evaluation, with a paramount emphasis on interference alignment. Such technique is specifically designed to cancel out the interference in scenarios where its level has the same magnitude as the desired signal. Furthermore, the main characteristics of the experiments are described, including the main features of the USRP devices, the spatial distribution of the nodes in the network under evaluation, and frame format to be transmitted by the different base stations, access points and user equipments. A selection of the obtained results are presented in terms of error vector magnitude, as a figure of merit to characterize the accuracy of the considered communication approaches. Finally, we establish a set of conclusions related to the experiments and the results mentioned above, and we incude a brief reflection on potential future lines to extend the research on this topic.Grado en Ingeniería de Tecnologías de Telecomunicació

Erratum to: Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) (Autophagy, 12, 1, 1-222, 10.1080/15548627.2015.1100356

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Multi-messenger Observations of a Binary Neutron Star Merger

International audienceOn 2017 August 17 a binary neutron star coalescence candidate (later designated GW170817) with merger time 12:41:04 UTC was observed through gravitational waves by the Advanced LIGO and Advanced Virgo detectors. The Fermi Gamma-ray Burst Monitor independently detected a gamma-ray burst (GRB 170817A) with a time delay of $\sim 1.7\,{\rm{s}}$ with respect to the merger time. From the gravitational-wave signal, the source was initially localized to a sky region of 31 deg(2) at a luminosity distance of ${40}_{-8}^{+8}$ Mpc and with component masses consistent with neutron stars. The component masses were later measured to be in the range 0.86 to 2.26 $\,{M}_{\odot }$. An extensive observing campaign was launched across the electromagnetic spectrum leading to the discovery of a bright optical transient (SSS17a, now with the IAU identification of AT 2017gfo) in NGC 4993 (at $\sim 40\,{\rm{Mpc}}$) less than 11 hours after the merger by the One-Meter, Two Hemisphere (1M2H) team using the 1 m Swope Telescope. The optical transient was independently detected by multiple teams within an hour. Subsequent observations targeted the object and its environment. Early ultraviolet observations revealed a blue transient that faded within 48 hours. Optical and infrared observations showed a redward evolution over ∼10 days. Following early non-detections, X-ray and radio emission were discovered at the transient’s position $\sim 9$ and $\sim 16$ days, respectively, after the merger. Both the X-ray and radio emission likely arise from a physical process that is distinct from the one that generates the UV/optical/near-infrared emission. No ultra-high-energy gamma-rays and no neutrino candidates consistent with the source were found in follow-up searches. These observations support the hypothesis that GW170817 was produced by the merger of two neutron stars in NGC 4993 followed by a short gamma-ray burst (GRB 170817A) and a kilonova/macronova powered by the radioactive decay of r-process nuclei synthesized in the ejecta